Alternative splicing in plants.

نویسندگان

  • Craig G Simpson
  • Dominika Lewandowska
  • John Fuller
  • Monika Maronova
  • Maria Kalyna
  • Diane Davidson
  • Jim McNicol
  • Dorota Raczynska
  • Artur Jarmolowski
  • Andrea Barta
  • John W S Brown
چکیده

The impact of AS (alternative splicing) is well-recognized in animal systems as a key regulator of gene expression and proteome complexity. In plants, AS is of growing importance as more genes are found to undergo AS, but relatively little is known about the factors regulating AS or the consequences of AS on mRNA levels and protein function. We have established an accurate and reproducible RT (reverse transcription)-PCR system to analyse AS in multiple genes. Initial studies have identified new AS events confirming that current values for the frequency of AS in plants are likely to be underestimates.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Role of Aberrant Alternative Splicing in Cancer

Alternative splicing can alter genome sequence and as a consequence, many genes change to oncogenes. This event can also affect protein function and diversity. The growing number of study elucidate the pathological influence of impaired alternative splicing events on numerous disease including cancer. Here, we would like to highlight the significant role of alternative splicing in cancer biolog...

متن کامل

Alternative splicing of pre-messenger RNAs in plants in the genomic era.

Primary transcripts (precursor-mRNAs) with introns can undergo alternative splicing to produce multiple transcripts from a single gene by differential use of splice sites, thereby increasing the transcriptome and proteome complexity within and between cells and tissues. Alternative splicing in plants is largely an unexplored area of gene expression, as this phenomenon used to be considered rare...

متن کامل

Alternative splicing and proteome diversity in plants: the tip of the iceberg has just emerged.

Alternative splicing has recently emerged as one of the most significant generators of functional complexity in several relatively well-studied animal genomes, but little is known about the extent of this phenomenon in higher plants. However, recent computational and experimental studies discussed here suggest that alternative splicing probably plays a far more significant role in the generatio...

متن کامل

Splicing-related genes are alternatively spliced upon changes in ambient temperatures in plants

Plants adjust their development and architecture to small variations in ambient temperature. In a time in which temperatures are rising world-wide, the mechanism by which plants are able to sense temperature fluctuations and adapt to it, is becoming of special interest. By performing RNA-sequencing on two Arabidopsis accession and one Brassica species exposed to temperature alterations, we show...

متن کامل

Genomewide comparative analysis of alternative splicing in plants.

Alternative splicing (AS) has been extensively studied in mammalian systems but much less in plants. Here we report AS events deduced from EST/cDNA analysis in two model plants: Arabidopsis and rice. In Arabidopsis, 4,707 (21.8%) of the genes with EST/cDNA evidence show 8,264 AS events. Approximately 56% of these events are intron retention (IntronR), and only 8% are exon skipping. In rice, 6,5...

متن کامل

Roles of Alternative Splicing for the Circadian Clock Control in Arabidopsis

Alternative splicing plays an important role in regulating gene functions and enhancing the diversity of the proteome in plants. Most of the genes are interrupted by introns in Arabidopsis. About one half of the intron-split genes involved in multiple biological processes including the circadian clock are alternatively spliced. In this review, we focus on the involvement of alternative splicing...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biochemical Society transactions

دوره 36 Pt 3  شماره 

صفحات  -

تاریخ انتشار 2008